Question

11.31 protein separation using affinity chromatography human insulin a and b from recombinant escherichia coli are separated using pilot-scale affinity chromatography. laboratory studies show that the capacity factors for the proteins are 0.85 for the a-chain and 1.05 for the b-chain. the dependence of hetp on liquid velocity satisfies the following type of equation h=4+ by + c where u is the linear liquid velocity and a, b, and c are constants. values of a. b, and c for the insulin system are 2x 10* m's", 15s, and 5.7x 10 m, respectively. two columns with inner diameter 25 cm are available for the process: one is 1.0 m high, the other is 0.7 m high (a) plot the relationship between h and u from u=0.1 x 10 ms to u-2x 10ms. (b) what is the minimum hetp? at what liquid velocity is the minimum hetp obtained? c) will the two insulins separate using the larger column consider that a value of rn bigger than 1.5 is for good resolution d) if the large column's flow is 0.31 limin, what will be the max flow for the smaller one?​

Answer 1

Respuesta:

k Hugo d k g

Explicación:

d g top top go f acaban tupido